Isolation and characterization of fetal nucleated red blood cells from maternal blood as a target for single cell sequencing‐based non‐invasive genetic testing
نویسندگان
چکیده
منابع مشابه
Isolation of fetal nucleated red blood cell from maternal blood using immunomagnetic beads for prenatal diagnosis.
The immunomagnetic beads method for isolation of fetal nucleated red blood cells (FNRBCs) from peripheral blood of 78 pregnant women for prenatal diagnosis was developed. The study subjects were classified into 8-10 and 11-14 weeks of gestation (n = 39 each). Peripheral blood cells were divided into two for the FNRBCs isolation using two protocols, one with anti-CD45 depletion followed by anti-...
متن کاملAutomated microscopy system for detection and genetic characterization of fetal nucleated red blood cells on slides
The detection and genetic analysis of fetal cells in maternal blood will permit noninvasive prenatal screening for genetic defects. Applied Imaging has developed and is currently evaluating a system for semiautomatic detection of fetal nucleated red blood cells on slides and acquisition of their DNA probe FISH images. The specimens are blood smears from pregnant women (9-16 weeks gestation) enr...
متن کاملDesign and Simulation of a Microscale Magnetophoretic Device for the Separation of Nucleated Fetal Red Blood Cells from Maternal Blood
Intense research has been carried out into methods that aim at harvesting fetal cells from maternal blood as substitutes to amniocentesis and chorionic villus sampling. Although much has been accomplished using well established techniques, a rapid and inexpensive method to separate fetal cells with great accuracy and efficiency to maximize cell yield is still required. This work focuses on the ...
متن کاملComparison of noninvasive prenatal testing of cell‐free DNA in maternal blood and amniocentesis for evaluation of aneuploidy
Background: The aim of this study was to compare noninvasive prenatal testing (NIPT) of cell‐free DNA in maternal blood and amniocentesis in the diagnosis of aneuploidy. This study was designed to evaluate sensitivity, specificity, accuracy, positive predictive value and negative predictive value of NIPT for detection of aneuploidies compared gold standard test of amniocentesis. Materials and m...
متن کاملThe Effects of Decreasing Maternal Anxiety on Fetal Oxygenation and Nucleated Red Blood Cells Count in the Cord Blood
OBJECTIVE Vasoconstriction during anxiety reduces fetal oxygenation and leads to hypoxia. Hypoxia in turn results in increase of the number of nucleated red blood cells (NRBCs) in the cord blood. The present study aimed to assess the effect of decreasing maternal anxiety on fetal oxygenation and NRBCs count in the cord blood. METHODS . In this study, 150 women were randomly divided into two i...
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ژورنال
عنوان ژورنال: Reproductive Medicine and Biology
سال: 2021
ISSN: 1445-5781,1447-0578
DOI: 10.1002/rmb2.12392